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Home-> Food Hygiene Detection Series-> Chromogenic Medium Series-> Urine Orientation Chromogenic Medium (UTI)

Urine Orientation Chromogenic Medium (UTI)

microbial medium
Chinese Name:尿道定位显色培养基
Product No.:HB7013
Specification:1000ml
Product Price:
Product Use:A chromogenic medium for the presumptive identification and differentiation of all the main micro-organisms that cause urinary tract infections
Remarks:

Technical Data:

Urine Orientation Chromogenic Medium (UTI) is a chromogenic medium for the presumptive

identification and differentiation of all the main micro-organisms that cause urinary tract infections 

Formula:

Ingredients

gm/litre

Peptic digest of animal tissue

15.0

Chromogenic mix

4.5

Agar

15.0

Final pH 7.0 ± 0.2 at 25℃


*Adjusted and/or supplemented as required to meet performance criteria.

Directions:
Suspend 34.5 grams of Urine Orientation Chromogenic Medium (HB7013) in 1 litre of distilled water. Mix well and sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to 50°C and pour into sterile Petri dishes.

Description:
Urine Orientation Chromogenic Medium contains two specific chromogenic substrates which are cleaved by enzymes produced byEnterococcus spp., E. coli and coliforms. In addition, it contains tryptophan which indicates tryptophan deaminase activity (TDA), indicating the presence of Proteus spp. It is based on Cystine Lactose Electrolyte Deficient (CLED) Medium which provides a valuable non-inhibitory diagnostic agar for plate culture of other urinary organisms, whilst preventing the swarming of Proteus spp.
The chromogen, X-glucoside, is targeted towards ß-glucosidase enzyme activity, and allows the specific detection of enterococci through the formation of blue colonies.
The other chromogen, Red-Galactoside, is cleaved by the enzyme ß-galactosidase which is produced by E. coli, resulting in pink colonies. Any uncertainty in identification may be resolved by removing suspect colonies from the plate and performing an indole test using Kovacs Indole Reagents (code HB8279).
The medium also contains tryptophan which acts as an indicator of tryptophan deaminase activity (TDA), resulting in halos around the colonies of Proteus, Morganella and Providencia spp.

Appearance:
Dehydrated Culture Medium is a free-flowing straw -colored powder. 
The prepared medium is a straw-colored, transparent agar.
Precautions:
Urine Orientation Chromogenic Medium should only be used for in vitro diagnostic purposes. 

Do not use beyond the stated expiry date, or if the product shows any sign of deterioration.

Storage conditions and Shelf life:
Urine Orientation Chromogenic Medium must be stored tightly capped in the original container at 10-30°C. 

The dehydrated medium has a shelf life of 3 years from date of manufacture. 

Prepared medium may be stored, out of direct light for up to 2 weeks at 2-8°C.

Quality control:

Test Organisms

Incubation

Results

Time

Temperature

Atmosphere

Escherichia coli

ATCC® 25922

24hr

36°C±1°C

Aerobic

Growth; 

medium sized rose to magenta colonies

with darker pink centers

Klebsiella pneumoniae

ATCC® 13883

24hr

36°C±1°C

Aerobic

Growth;

large, deep blue or dark indigo colonies

Enterococcus faecalis

ATCC® 29212

24hr

36°C±1°C

Aerobic

Growth;

small, teal to turquoise colonies

Proteus mirabilis

ATCC® 12453

24hr

36°C±1°C

Aerobic

Growth; clear to light yellow colonies

with golden-orangecolor diffused 

through surrounding media

Staphylococcus aureus

ATCC® 25923

24hr

36°C±1°C

Aerobic

Growth; opaque, cream to 

white colored colonies

Pseudomonas aeruginosa

ATCC® 27853

24hr

36°C±1°C

Aerobic

Growth; colorless to light yellow-green,

translucentcolonies, which may have

a slight iridescence

Staphylococcus 

saprophyticus

ATCC® 15305

24hr

36°C±1°C

Aerobic

Growth; opaque, pink colonies

Candida albicans

ATCC® 10231

24hr

36°C±1°C

Aerobic

Growth; small, white,moist colonies

Citrobacter freundii

ATCC® 8090

24hr

36°C±1°C

Aerobic

Growth; 

dark blue colonies, often with 

a rose halo in the surrounding media

Reference:

1.Collee J. G., Fraser A. G., Marmion B. P., Simmons A., (Eds.), Mackie and cCartney, Practical Medical Microbiology,1996, 14th Edition, Churchill Livingstone.

2.Pezzlo M., 1998, Clin. Microbiol. Rev., 1:268-280.

3.Wilkie M. E., Almond M. K., Marsh F. P., 1992, British Medical Journal 305:1137-1141.

4.Friedman M. P. et al, 1991, J. Clin. Microbiol., 29:2385-2389.

5.MurrayP., Traynor P. Hopson D., 1992, J. Clin. Microbiol., 30:1600-1601.

6.Soriano F., Ponte C., 1992, J. Clin. Microbiol., 30:3033-3034.

7.Merlino et al, 1995, Abstr. Austr. Microbiol. 16(4):17-3.



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