culture medium
Home-> Food Hygiene Detection Series-> Chromogenic Medium Series-> Shigella Chromogenic Medium

Shigella Chromogenic Medium

microbial medium
Chinese Name:志贺氏菌显色培养基
Product No.:HB7018
Specification:1000ml
Product Price:
Product Use:Specially used for the rapid detection of Shigella in food and patient stool samples. The colonies of Shigella are white or colorless, some sonnei Shigella have blue center
Remarks:

Technical Data:

Shigella Chromogenic Medium is specially used for the rapid detection of Shigella in food and patient stool samples. The colonies of Shigella are white or colorless, some sonnei Shigella have blue center; Escherichia coli and Coliforms have green or yellow colonies; salmonella are yellow or colorless and have a black center; other are blue-green or yellow. Proteus and Pseudomonas aeruginosa basically do not grow; it is an ideal culture medium for rapid detection.

Ingredients (g/L):

Special Nutrients

70.2

Chromogenic reagent

0.4

Agar

15.0

Final pH 7.3±0.1 at 25℃

*Adjusted and/or supplemented as required to meet performance criteria

Directions:

Suspend 17.12g of the medium in 200mL of distilled water, heat in 1 minute. When cooling to 45-50 ℃, pour into sterile petri dishes.

Warning: 

Prepared medium must be kept dry, can place inverted culture in incubator at 36 ± 1 ℃ for 1-2 hours.

Procedures:

1. Prepared sample solution according to national standards, SN standard, FDA standards or other methods.

2. Transfer 25g sample solution into 225mL GN Broth or Shigiella Broth, enrichment culture for 18- 24hours at 36 ± 1 ℃;

3. Take one ring enrichment broth with a 3mm inoculation loop, streak to inoculated o Shigella Chromogenic Medium, make two plates

4. Culture at 36 ± 1 ℃ for 20-24 hours, the colonies of Shigella are white or colorless, some sonnei Shigella have blue center; Escherichia coli and Coliforms have green or yellow colonies; salmonella are yellow or colorless and have a black center; other are blue-green or yellow. Proteus and Pseudomonas aeruginosa basically do not grow

5.  For typical colonies, do Shigella serological test and full set of biochemical tests.

Quality Control:

1. Appearance:

The dehydrate powder medium is homogeneous, having good flowability, grey white to pale pink, prepared medium is red solid.

2. Microbial sensitivity test:

Incubated at 36 ± 1 ℃ 18-24h:

QC Strains

Strains No.

Growth

Colonies color

Shigella flexneri

ATCC12022

+++

Colorless or white, 

the diameter of colonies is 1-3mm

Shigella dysentery

CMCC51252

+++

Colorless or white,

 the diameter of colonies is 1-3mm

Shigella boydii

CMCC51582

+++

Colorless or white, 

the diameter of colonies is 1-3mm

sonnei Shigella

CMCC51592

+++

Colorless or white, the diameter of 

colonies is 1-3mm, with blue center

Staphylococcus aureus

ATCC25923

-

/

E. coli

ATCC25922

+++

green

salmonella

ATCC14028

+++

Colorless with black center

Proteus mirabilis

ATCC35029

-/+

Colorless, diameter<1mm

Klebsiella pneumoniae

ATCC10031

++

Colorless with black center

Freundii Citrobacter

ATCC43864

+++

Colorless with black center

Pseudomonas aeruginosa

CICC10211

-/+

Colorless, diameter<1mm

Methods limitations:

The typical Shigella on t medium must be do serological test and biochemical tests

Typical features:

The colonies of Shigella are white or colorless, some sonnei Shigella have blue center; Escherichia coli and Coliforms have green or yellow colonies; salmonella are yellow or colorless and have a black center; other are blue-green or yellow. Proteus and Pseudomonas aeruginosa basically do not grow.

Storage:

The prepared plates can be kept for 2-5 days, avoid direct exposure to light. The powder medium should be placed in a dark, dry place, storage temperature 2-8 ℃, avoid direct exposure to light.

Failure:

Dry powder media which are over the shelf life, caking and color variation cannot be used.

Note:

This medium is only for laboratory use.

Reference:

1. Restaino, L., E. W. Frampton, W. C. Lionberg, and A. L. Restaino. 2007. Detection of Shigella sp.using a selective and differential chromogenic plating medium. Abstract P3-43. IAFP.


Shigella Chromogenic MediumRelated products:

Product numberProduct nameProduct specificationDirections
HB0114-1VRB-MUG Agar
VRB-MUG琼脂
100gUsed for solid plate detection for E. coli (GB standard)
HB0141Aliz-gal Agar
茜素-β-半乳糖苷琼脂(Aliz-gal琼脂)
100gfor the rapid detect and count coliform in food, beverage and drinking water (GB / T)
HB5213MUG Medium
MUG培养基
100gDetection of Escherichia coli
HB7001E.Coli Chromogenic Medium
大肠杆菌显色培养基
1000mlFor fast, accurate detection of E. coli in food, water, milk, ice cream and meat culture for 24 hours, the colonies of E. coli are Blue-green
HB7002Coliforms Chromogenic Medium
大肠菌群显色培养基
1000mlFor fast, accurate detection of coliforms in food, water, milk, ice cream and meat, culture for 24 hours, the colonies of coliforms are blue-green
HB7003E.Coli & Coliforms Chromogenic Medium
大肠杆菌/大肠菌群显色培养基(第二代)
1000mlUsed for fast, accurate simultaneous detection of E. coli and Coliform bacteria, incubated for 24 hours, E. coli was blue-green - purple, coliform is red
HB7004TBX Medium
TBX培养基
1000mlFor fast, accurate detection of E. coli in food, water, milk, ice cream and meat culture for 24 hours, the colonies of E. coli are Blue-green
HB7005Total Genes Chromogenic Medium
细菌总数显色培养基
250g/500gFor fast, accurate detection of total bacterial count in food, water and the environment, culture for 24 hours, bacterial colonies are red.
HB7006E.Coli O157:H7 Chromogenic Medium
O157显色培养基
1000mlFor rapid detection. E.Coli O157: H7 in food and Patient faecal samples. The colonies are brilliant red, pink or red, no blue halo around
HB7007-1Salmonella Chromogenic Medium (GenerationⅡ)
沙门氏菌显色培养基(第二代)
1000mlFor the rapid detection of food, water, milk, ice cream and meat and other samples of Salmonella. The colonies of salmonella are purple
HB7008Listeria Chromogenic Medium
李斯特氏菌显色培养基
1000mlUsed for rapid detection of Listeria monocytogenes in food and medicine. The colonies of Listeria monocytogenes is blue, surrounded by an opaque ring
HB7009Staphylococcus aureus Chromogenic Medium
金黄色葡萄球菌显色培养基
1000mlFor food, rapid detection of Staphylococcus aureus in water, milk, ice cream and meat products, within 24 hours. The colonies of Staphylococcus aureus are blue-green
HB7010-1Mold and Yeast Chromogenic Medium
霉菌和酵母菌显色培养基
1000mlFor chromogenic cultivate of mold and yeast
HB7011Vibrio Chromogenic Medium
弧菌显色培养基
1000mlUsed for the rapid detection of Vibrio parahaemolyticus in food, seafood, and fish in patient stool samples.
HB7012Enterobacter Sakazakii Chromogenic Medium
阪崎杆菌显色培养基
1000mlUsed for rapid detection of infant formula and other food products. The colonies of Enterobacter sakazakii are blue-green colonies
HB7013Urine Orientation Chromogenic Medium (UTI)
尿道定位显色培养基
1000mlA chromogenic medium for the presumptive identification and differentiation of all the main micro-organisms that cause urinary tract infections
HB7014-1Enterococcus Chromogenic Medium
肠球菌显色培养基
1000mlUsed for rapid detection of food, water and environment, Enterococcus is red to purple colonies,
HB7015Candida Chromogenic Medium
念珠菌显色培养基
1000mlUsed for the isolation and identification of Candida, the colonies of Candida albicans are blue-green
HB7021-2Bacillus Cereus Chromogenic Medium (Generation Ⅲ)
蜡样芽孢杆菌显色培养基(第三代)
1000mlUsed for the rapid detection of Bacillus cereus in food, water, dairy products and meat products.
HB8499MUGal Broth
MUGal肉汤
100gFor rapid detection of coliforms counts in food, beverage and drinking water
HB8558-1MUG Nutrient Agar(NA-MUG)
MUG营养琼脂(NA-MUG)
100gUsed for detection Escherichia coli in drinking water and water resource by filter membrane method.
HB8558MUG Nutrient Agar(NA-MUG)
MUG营养琼脂(NA-MUG)
250g/500gUsed for detection Escherichia coli in drinking water and water resource by filter membrane method.
HB8559-1EC-MUG Medium
EC-MUG培养基
100gFor the detection of E. coli in drinking water and water resource.
HB8559EC-MUG Medium
EC-MUG培养基
250g/500gFor the detection of E. coli in drinking water and water resource.
HB8569-1MMO-MUG Medium
MMO-MUG培养基
250g/500gFor synchronously test coliform and E.coli in drinking water and water resource.
HB8569-2MMO-MUG Medium(100g)
MMO-MUG培养基(100g)
100gFor coliforms and Escherichia coli testing in drinking water and water resource
HB8686MI Medium
MI培养基
1000mlUsed for Escherichia coli and coliform bacteria count in drinking water by membrane filtration method.
HB8691MEI Medium
MEI培养基
1000mlUsed for chromogenic detection by one-step membrane filtration or count of enterococci in water
    
Comments Box
product of interest: *
Quantity: *
Name: *
Email: *
Address:
TEL:
Message:
*
Verification Code: * Verification Code
      
Latest Product
 mCPC Medium
 Amies Transport Medium Tube(Liquid)
 Virus Transport Medium (VTM)
 Stuart Transport Medium
 Medium 19
 Medium 3
 
Recommended Products
 Freeze-Dried Rabbit Plasma
 
catalog
Home | Profile | Add to Favorites | Online service | Contact
Address: No.1, jinhui Road, Gaoxin District, Qingdao City shandong china
TEL:400-0532-596 +86 15318781306
+86 18753251568

Fax:0532-81935080 66087775

Email:andy@hopebiol.com

alfred@hopebiol.com

QQ:876717509; 1063178955

Complaints and suggestions
Tel:13105190021 13006536294
Email:hopebiol@163.com
©2001- Copyright Qingdao Hope Bio-Tcehnology Co.,Ltd