culture medium
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TCBS Agar Granule

microbial medium
Chinese Name:TCBS琼脂颗粒
Product No.:HBKD4130
Specification:300mL/pack*10packs
Product Price:
Product Use:Used for the selective isolation of Vibrios from a variety of clinical material, water and foods.
Remarks:

Technical Data:

Approximate Formula(gm/liter):

Yeast Extract

5.0

Sodium Chloride

10.0

Proteose Peptone

10.0

Ferric Ammonium Citrate

1.0

Sodium Thiosulfate

10.0

Bromothymol Blue

0.04

Sodium Citrate

10.0

Thymol Blue

0.04

Oxgall

8.0

Agar

15.0

Saccharose

20.0

Final pH 8.6±0.2 at 25

*Adjusted and/or supplemented as required to meet performance criteria.

Directions:

Suspend89.0 gof the medium in one liter of distilled water or deionized water. Heat with frequent agitation and boil for 1 minute. Cool to 45-50and pour in Petri dish. DO NOT AUTOCLAVE.

Principle and Interpretation:

TCBS Agar is highly selective for the isolation of Vibrios. Bile salts inhibits the growth of gram-positive bacteria. Sodium thiosulfate serves as a sulfur source and, in combination with ferric ammonium citrate, detects hydrogen sulfide production. Saccharose is included as a fermentable carbohydrate for the metabolism of Vibrios. The alkaline pH of the medium enhances the recovery of Vibrios cholerae. Thymol blue and bromthymol blue are included as indicators of pH changes.

Appearance:

Dehydrated medium is a free-flowing light grey granule.The prepared medium is a kind of blue-green gel.

Precautions:

This medium is for laboratory use only. Dried medium which is past shelf life, caking or color variation cannot be used.

Storage conditions and Shelf life:

TCBS Agar must be stored tightly capped in the original container at 5-30°C. The dehydrated medium has a shelf life of 3 years from date of manufacturing. Prepared medium may be stored, out of direct light at 2-8°C.

Quality control:

Inoculate and incubate at 36±1℃for 18-24 hours.

Microorganism

Strains Number

Inoculum (CFU

Growth

Recovery

Remarks

Vibrio parahaemolyticus

ATCC 17802

20-200

Luxuriant

20%

Blue-green bamboo hat-like colonies, H2S-

Vibrio alginolyticus

ATCC 33787

20-200

Luxuriant

20%

Yellow colonies, H2S-

Escherichia coli

ATCC 25922

100-1000

Inhibited

< 1%

/

Staphylococcus aureus

ATCC 25923

100-1000

Inhibited

< 1%

/

Proteus vulgaris

ATCC 13315

100-1000

Poor/ Ihibited

< 10%

Center black and edge yellow colonies, H2S +

Reference

1. GB4789.28-2013 People's Republic of China food safety national standard food microbiology test medium and reagent quality requirements.

2. GB/T5750.12-2006 People's Republic of China Standard examination Methods for drinking water--Microbiological parameters.


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